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. Author manuscript; available in PMC: 2012 Aug 28.
Published in final edited form as: Science. 2009 Nov 13;326(5955):991–994. doi: 10.1126/science.1175326

Fig. 1.

Fig. 1

Prophase I arrest is compromised after BubR1-depletion. (A) Schematic of MI. (B) Mock-depleted (+ControlMO), BubR1-depleted (+BubR1MO), Mad2-depleted (+Mad2MO)(7), Cdh1-depleted (+Cdh1MO)(9) and uninjected oocytes were scored for GVBD after 24 h in IBMX. Note that Cdh1-depleted oocytes escape prophase I arrest as shown previously(2). Increased GVBD following BubR1-depletion could be prevented by expressing either BubR1 from an hBubR1 cRNA (+BubR1MO+hBubR1 cRNA) or Cdh1 from a Cdh1 cRNA (+BubR1MO+Cdh1 cRNA)(9). Error bars, mean ± SEM; N ≥ 3. Asterisks denote a significant difference from uninjected controls (P < 0.0001; Student’s t-test). (C and D) Samples (50 oocytes) of GV-stage oocytes from each of the groups depicted were immunoblotted either for Cdh1 and actin (C) or for BubR1, Cdh1 and GAPDH (D) (N = 2). (*), non-specific band.