Fig. 2.

Effect of prolonged ovalbumin (OVA) challenge on matrix deposition. (a) Image analysis of Martius scarlet blue-stained lung sections from mice treated with alum or OVA during acute and prolonged allergen challenge. Random measurements from the basement membrane into the submucosa (10 measurements of 20 μm in length) were taken and the mean density calculated from four bronchioles per mouse, n = 4 mice per group. (b) Levels of total collagen were measured in lung homogenate samples taken throughout the model. Data is expressed as mean ± SEM, n = 4/group; *P<0.05 in comparison with alum control mice.