Fig. 1.

T. muris eggs are induced to hatch in vitro by contact with bacteria. (A) Hatching of T. muris eggs for 2 hours at 37°C incubated with 5-cm sections of mouse cecum, E. coli bacterial suspension, or 0.22-μm filtered (overnight) bacterial suspension. Luria-Bertani (LB) broth was used as negative control. (B) T. muris eggs were cultured with four strains of bacteria (E. coli, S. aureus, S. typhimurium, or P. aeruginosa) for 2 hours at 37°C. (C) T. muris eggs incubated with Saccharomyces cerevisiae, with or without 0.4-μm transwell or 0.22-μm filtered S. cerevisiae cultures for 2 hours at 37°C. (D) Hatching of T. muris eggs in E. coli bacterial suspension or with 0.4-μm or 3-μm transwells. (E) T. muris eggs incubated for 2 hours with E. coli at 4°C, room temperature (RT), or 37°C. Eggs that did not hatch at 4°C or room temperature were then incubated for a further 2 hours at 37°C (4/37 and RT/37, respectively). (F to H) T. muris eggs were cultured at 37°C for 1 hour with GFP-expressing bacteria, then washed in phosphate-buffered saline before further incubation for 1 hour at 37°C. (F) Light-field image; (G) fluorescence image; (H) combined image. Scale bar, 10 μm. All figures show means ± SEM, *P < 0.01, using analysis of variance (ANOVA).