Fig. 3.

Western blot analysis of purified FimH binding to surface egg proteins. T. muris eggs are induced to hatch in vitro after contact with FimH adhesion on type 1 fimbriae. Egg proteins were subjected to SDS–polyacrylamide gel electrophoresis under reducing conditions and electrophoretically transferred onto nitrocellulose. The migration positions of protein standards are indicated on the left. Lane 1, purified FimH and secondary antibody; lane 2, purified FimH preincubated with mannose to specifically block binding; lane 3, purified FimH only (no secondary); lane 4, secondary only.