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. Author manuscript; available in PMC: 2012 Aug 28.
Published in final edited form as: Endocrinology. 2011 Oct 11;152(12):5029–5040. doi: 10.1210/en.2011-1124

Figure 4. Nuclear translocation of p65 or IκBα degradation in granulosa cells was induced by TNFα but not LPS.

Figure 4

Granulosa cells were cultured in glass bottom petri dishes and transfected with IκBα-EGFP (a-b, e-f) and p65-dsRed (c-d, e-h). Live cell confocal microscopy under culture conditions was performed to track degradation of IκBα-EGFP and nuclear translocation of p65-dsRed. Cells were treated with 20 ng/ml TNFα (a-d) as a positive control, or 1 μg/ml ultrapure LPS (e-h). Scale bar represents 50μm.