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Purification of C. fasciculata TST
| Fraction | Activity (U/ml) |
Total Protein (mg) |
Total Units (mU) |
Specific Activity (mU mg−1) |
Purification factor |
Recovery (%) |
|---|---|---|---|---|---|---|
| Crude extract | 510 | 19,880 | 145,410 | 7.3 | - | 100 |
| 4-9% PEG | 640 | 2,010 | 63,110 | 31.4 | 4.3 | 43 |
| Q-sepharose | 770 | 72 | 58,239 | 815 | 112 | 40 |
| Phenyl sepharose | 2,080 | 9.1 | 13,960 | 1,530 | 210 | 9.6 |
| Superdex 200 | 4,140 | 5 | 8,290 | 1,658 | 227 | 5.7 |
