. Author manuscript; available in PMC: 2012 Aug 28.

Published in final edited form as: J Biol Chem. 2004 Apr 8;279(26):27246–27256. doi: 10.1074/jbc.M311039200

Table 3.

Kinetic parameters of the C. fasciculata TST

Substrate ^a)	K_m(app) (μM)	V_max (nmol min⁻¹)	k_cat (s⁻¹) ^f)	k_cat/K_m (M⁻¹ s⁻¹)
CDNB ^b)	190± 27	3.45± 0.14	236	1.2 × 106
T(SH)₂ ^c)	159± 19	2.77± 0.12	190	1.2 × 106
GspdSH ^d)	310± 31	2.81± 0.13	193	0.62 × 106
GSH monoethyl ester ^e)	6,100± 590	0.59± 0.02	17.4	0.003 × 106

^a)

The absorbance coefficient for the T[DNB]₂ conjugate of 9.2 mM cm⁻¹ was used for both T[SH]₂ and GspdSH, whilst the published value for GS-DNB of 9.6 mM cm⁻¹ was used for GSH ester.

^b)

Measured with 400 μM T[SH]2 as the fixed substrate

^c)

Measured with 400 μM CDNB as the fixed substrate

^d)

Measured with 400 μM CDNB as the fixed substrate

^e)

Measured with 400 μM CDNB as the fixed substrate

^f)

Calculated using the calculated concentration of the [(αβγ₂)]₄ complex