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. 2012 Aug 27;209(9):1529–1535. doi: 10.1084/jem.20112646

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© 2012 Schlenner et al.

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Figure 1. — Deletion of Smad-binding site in foxp3 enhancer/CNS1. (A) Schematic depiction of upstream region of wild-type foxp3 locus and the gene targeting vector. The CNS1 and the position of the Smad-binding site in the wild type or deleted binding site in the foxp3^CNS1mut allele are indicated. (B) Alignment of the wild type and the foxp3^CNS1mut allele at the position of the Smad-binding site, showing the deleted sequence. (C) ChIP analysis of Smad3 binding to the foxp3 CNS1 region. foxp3^WT (sample 1) or foxp3^CNS1mut (sample 2) CD4⁺CD25⁻ splenocytes were not stimulated (0 h) or stimulated for 1 h with TGF-β, anti-CD3, and anti-CD28. Immunoprecipitated DNA was analyzed by PCR. Data are representative of two independent experiments.