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. 2012 Aug 27;209(9):1529–1535. doi: 10.1084/jem.20112646

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© 2012 Schlenner et al.

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Figure 4. — Analysis of T reg cell frequencies, proliferation rate, and proportion of Helios-expressing T reg cells in adult males. 4-wk-old (A), 8-wk-old (B), and 6-mo-old (C) males have been injected with 1 mg EdU i.p. 16 h before analysis. (A and B) Thymi (thy), spleen (spl), axial/inguinal LN, MLN, PP, LPL, and IEL have been analyzed for frequency of Foxp3⁺ T reg cells among CD4⁺ T cells (top), EdU⁺ (middle), and Helios⁺ (bottom) cells among T reg cells. Data are representative of two to six males and two to three independent experiments per group. (C) Frequencies of T reg cells (left) and EdU⁺ T reg cells (right) were assessed in spleen, PP, LPL, and IEL. Data are representative of 7–10 males and five independent experiments per group. Data for foxp3^WT mice are depicted in black and data for foxp3^CNS1mut mice are depicted in gray. Error bars indicate means ± SEM. *, P < 0.03.