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. 2012 Aug 27;209(9):1519–1528. doi: 10.1084/jem.20120189

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© 2012 Venereau et al

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Figure 4. — HMGB1 cysteines are essential to promote cytokine/chemokine production, but not for chemotaxis. (A) Electrophoretic mobility of recombinant WT HMGB1 and mutants prepared in the absence of DTT, determined as described in the legend of Fig. 1 B. (B) Human macrophages were stimulated for 4 h with mutants or WT HMGB1 prepared in the absence of DTT (0.4 µM). TNF expression was measured by real-time PCR (*, P < 0.05 vs. control, ANOVA). (C and D) 3T3 fibroblast migration toward 1 nM HMGB1 mutants, exposed or not for 1 h to 5 mM DTT (C) or to 100 mM H₂O₂ (D). Bars represent the mean ± SD of triplicate samples (*, P < 0.05 vs. control, ANOVA).