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. 2012 Aug 27;209(9):1641–1653. doi: 10.1084/jem.20110306

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© 2012 Banerjee et al.

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Figure 6. — Restoration of normal thymic development and peripheral T cell phenotype in Hq mice requires the oxidoreductase function but not the DNA-binding function of Aif. (A) Western blot analysis of Aif expression in spleen cell lysates. Mice bearing transgenes encoding the mutant Aif constructs Aif254/264 or Aif262/299 were bred with heterozygous Hq females to generate male tg WT and tg Hq littermates as shown, and spleen cell lysates were tested. (B–E) Frequencies of naive CD4 (B and D) and naive CD8 (C and E) cells in spleens from 6–8-wk-old WT and Hq littermate mice of either non-tg or tg genotypes (with either Aif254/264 or Aif262/299 as indicated) as shown, as mean ± SE (n = 3). *, P < 0.01. (F–I) Numbers per spleen of naive CD4 (F and H) and naive CD8 (G and I) cells in WT and Hq littermate mice of either non-tg or tg genotypes (with either Aif254/264 or Aif262/299 as indicated) as shown, as mean ± SE (n = 3). *, P < 0.01. (J and K) Numbers of thymocytes from 6–8-wk-old WT and Hq littermate mice of either non-tg or tg genotypes (with either Aif254/264 or Aif262/299 as indicated) as shown, as mean ± SE (n = 3). *, P < 0.01. (L–Q) Frequencies of DN (L and M), DN3 (N and O), and DN4 (P and Q) thymocytes from WT and Hq littermate mice of either non-tg or tg genotypes (with either Aif254/264 or Aif262/299 as indicated) as shown, as mean ± SE (n = 3). *, P < 0.01. All flow cytometric analyses are representative of at least two independent experiments.