Table 3. Immunohistochemical methods used for the different fixatives.
| Primary antibody | Antigen | Dilution | Antigen retrieval (AR) | Fixative* |
|---|---|---|---|---|
| PAN-CK | Epithelial cells | 1:200 | Protease XIV 10 min | F, B, H |
| CD31 | Endothelial cells | 1:50 | 3 Cycles Tris/EDTA 1 Cycle Tris/EDTA |
F, B, H G, U, C |
| Ki-67 | Ki-67 antigen | 1: 50 | Citrate buffer | A |
| S100 | S100 protein | 1: 700 | No AR needed | A |
| CD68 | Macrophages | 1: 50 | Protease XIV 10 min | A |
| AML | Actin (Smooth Muscle) | 1: 100 | No AR needed | A |
*
F, formalin; B, Bouin; H, Hollande; G, Greenfix; U, UPM; C, CyMol; A, All fixatives.