Table 5. Colour contrast analysis of the histochemical staining.
| Colour contrast (mean±ds) | |||||||
|---|---|---|---|---|---|---|---|
| Staining | M | Formalin | Hollande | Bouin | Greenfix | UMP | CyMol |
| H&E | r | 174.36±8.67 | 187.12±3.33* | 200.77±3.71* | 177.77±2.18 | 198.2±6.34* | 184.5±9.95 |
| g | 163.997±9.21 | 159.67±3.68* | 163.88±3.43 | 171.66±2.63 | 175.94±4.74 | 160.44±10.7 | |
| b | 177.07±10.39 | 189.03±3.06 | 195.88±4.15* | 180.44±3.97 | 190.71±8.97 | 180.36±12.56 | |
| PAS | r | 224.95±8.88 | 186.88±13.94* | 201.88±8.28* | 211.48±5.61 | 193.87±6.78* | 187.94±4.45* |
| g | 204.76±17.22 | 150.44±24.35* | 152.25±11.97* | 196.51±16.76* | 167.3±14.09* | 120.55±16.17* | |
| b | 229.73±8.66 | 204.41±10.32* | 219.37±6.74 | 233.91±3.53 | 208.62±5.22* | 200.77±6.81* | |
| Alcian | r | 195.97±12.73 | 209.71±6.36 | 201.9±5.22 | 194.77±2.46 | 199.95±4.61 | 198.13±1.92 |
| g | 196.63±10.98 | 198.69±6.1 | 191.3±4.92 | 184.25±2.38 | 195.06±5.51 | 197.04±1.63 | |
| b | 217.41±5.43 | 204.77±6.72 | 200.42±3.37 | 187.6±2.6* | 199.03±3.94* | 201.41±1.52* | |
| Trichrome | r | 200.72 ±13.65 | 203.88±8.81 | 161.59±4.45* | 192.5±7.6 | 120.03±25.19* | 147.4±11.65* |
| b | 208.11±11.22 | 205.99±12.56 | 215.36±1.1 | 193.07±9.07 | 167.18±21.79* | 152.98±10.61* | |
| b | 215.57±6.55 | 219.44±8.63 | 210.39±0.95 | 212.27±3.96 | 189.57±13.67* | 167.78±9.97* | |
| HID | r | 157.68±3.85 | 160.14±6.28 | 155.26±3.12 | 157.33±1.88 | 150.13±2.43* | 160.01±1.54 |
| g | 160.54±2.28 | 166.92±4.8 | 155.2±1.97* | 156.72±3.34 | 157.43±1.22 | 164.23±1.64* | |
| b | 161.46±2.71 | 167.07±2.94* | 156.02±2.16* | 155.2±2.63* | 157.63±0.97 | 163.22±1.62 | |
The colour contrast of each slide was measured automatically using the colour histogram analysis of the ImageJR computer program. HID, high iron diamine; M, colour histogram mean; r, red; g, green; b, blue. Colour scale: values 0–255, with 0, maximum value; 255, minimum value.
*
P<0.05.