. 2012 May 4;56(2):e12. doi: 10.4081/ejh.2012.e12

Table 6. Colour intensity analysis of the immunohistochemical staining.

Immunostaining	Colour intensity (mean±ds)
	CDP	Formalin	Hollande	Bouin	Greenfix	UMP	CyMol
CK-PAN	1	247.66±3	241.95±5.27	220.01±14.31^*	244.65±0.62	242.58±3.3	257.67±14.22
	2A	120.68±9	102.58±10.42^*	158.73±4.3^*	64.74±6.21^*	95.65±7.09^*	95.65±13.47^*
	2B	239.14±2.58	232.22±6.44	217.25±6.18^*	231.77±6.29	250.42±1.65^*	251.41±2.88^*
Ki-67	1	220.37±2.17	211.57±2.75^*	208.94±20.03^*	204.78±7.17^*	217.68±1.78	214.38±2.55^*
	2A	79.14±12.33	54.11±2.8^*	51.24±23.99	59.15±10.55^*	124.68±26.51^*	88.69±3.33
	2B	230±10.24	225.34±5.83	225.18±2	212.3±5.17^*	208.91±5.05^*	213.24±5.29^*
CD31	1	177.41±3.43	182.61±3.92	165.86±4.07^*	176.17±2.01	179.43±3.82	163.47±9.37^*
	2A	57.84±8.93	89.88±3.12^*	36.44±8.02^*	32.31±3.68^*	27.54±6.24^*	31.76±8.06^*
	2B	183.78±3.29	179.46±2.33	171.51±6.49^*	177.32±7.37	181.25±1.52	158±9.81^*
CD68	1	224.82±1.89	206.75±4.71^*	194.09±3.84^*	226.46±3.96	223.23±3.25	239.79±2.43^*
	2A	52.88±14.21	26.9±7.65^*	20.86±4.64^*	12.29±2.23^*	23.31±4.83^*	23.34±8.63^*
	2B	211.85±7.3	190.82±9.29^*	162.24±15.39^*	138.38±3.82^*	146.39±8.61^*	139.55±9.68^*
AML	1	167.27±7.38	166.19±1.07	172.2±1.76	170.01±2.14	177.41±3.29^*	184.85±4.25^*
	2A	37.897±9.048	41.37±1.17	42.17±1.22	16.67±1.01^*	13.46±3.94^*	19.28±4.37^*
	2B	195.39±1.95	177.46±0.8^*	179.52±1.12^*	177.52±0.68^*	177.39±1.36^*	187.72±1.85^*
S100	1	202.09±3.49	156.26±51	219.66±1.97^*	206.69±0.87^*	200.65±0.32	214±9.85
	2A	18.35±3.32	21.64±0.58	21.77±0.93	48.88±5.66^*	21.56±2.16	24.26±1.11^*
	2B	214.22±10.98	222.29±0.96	220.46±1.18	215.76±4.26	183.32±9.94^*	222.39±1.77

Digital images were prepared for analysis using the ImageJ^R computer program. Colour intensity of each image was measured automatically using a colour deconvolution plugin (CDP) specific for immunohistochemistry. 1, haematoxylin; 2A, specific signal; 2B, background. Colour scale: values 0–255, with 0, maximum value; 255, minimum value.

P<0.05.