Figure 5.

No effect of DHA on ROS levels in isolated rabbit ventricular myocytes. ROS levels were measured by monitoring DCF fluorescence intensity in isolated myocytes every 30 s in control, H₂O₂ (200 μM) and H₂O₂ + DHA (25 μM) groups. (A) Time courses of DCF fluorescence intensity (F/F₀) in three representative myocytes from the three groups, respectively. (B) Histograms summarizing the DCF intensities for each group measured at 6 min after treatment of H₂O₂. **p < 0.01 compared to control. Numbers in parentheses indicate the number of cells in each group.