Figure 2.

(A) Photographs of atrial myocytes of zebrafish and human enzymatically isolated as described in detail previously (Verkerk et al., 2009a), except that the isolation temperature in the zebrafish procedure was decreased to 28°C. (B) Typical action potentials (APs) of a single zebrafish and human atrial myocyte measured at 1 Hz. Zebrafish and human atrial APs were recorded at 28 and 36°C, respectively [See Verkerk et al., 2009a (zebrafish) and Verkerk et al., 2007 (human) for solutions used]. (C) Average AP characteristics of zebrafish and human atrial myocyte recorded at 1 Hz. RMP, resting membrane potential; APA, AP amplitude; Vmax, maximal AP upstroke velocity; APD₂₀, APD₅₀, and APD₉₀, AP duration at 20, 50, and 90% repolarization, respectively. Values are mean ± SEM; ^*P < 0.05 (t-test).