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IHC showed expression and co-localization of GAD67 and BDNF in the ventral horn (VH) motoneurons (arrows). GAD67 showed a diffuse staining in cell bodies and fibers as well as punctate staining in the VH (A). Interestingly, those GAD67 immunostained motoneuron cell bodies also stained with BDNF (B); (C) is the merged image of (A,B) (20× magnification).
