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Compromised Akt activation in response to insulin in the liver (A), epididymal adipose tissue (B), and heart (C) of mPer2Brdm1 mice. Representative immunoblotting and respective quantifications of Akt-S473 phosphorylation upon intraperitoneal injection of insulin (0.5 mU/g body weight, 5 min) at ZT9 and ZT15 (n = 7−10), respectively. The symbol “−” or “+” means without or with insulin stimulation, respectively. *p < 0.05 and **p < 0.01 between the indicated groups.
