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. Author manuscript; available in PMC: 2012 Aug 28.

Published in final edited form as: Leukemia. 2008 May 29;22(8):1595–1603. doi: 10.1038/leu.2008.129

A) Western blots revealed that SU-DHL-1 cells treated with IL-22BP (5 μg/mL) or IL-22 neutralizing antibody (10 μg/mL) resulted in a gradual and time-dependent decrease in pSTAT3. On the right panel, densitometry results for pSTAT3 were included.

B) Western blot revealed that SU-DHL-1 cells treated with recombinant IL-22 (10 ng/mL) for 30 minutes increased the level of pSTAT3. Three other signaling proteins in the MAPK kinase pathway (ERK1/2, JNK/SAPK, p38) were also activated. On the right panel, densitometry results for pSTAT3, pJNK/SAPK, pERK1/2, and phoshpho-p38 were included.

C) Compared to the scrambled siRNA, IL-22R1 siRNA downregulated both IL-22R1 and pSTAT3 (cell lysates prepared 24 hours after transfection). The STAT3 and β-actin expression levels were equal in both lanes.

A) Western blots revealed that SU-DHL-1 cells treated with IL-22BP (5 μg/mL) or IL-22 neutralizing antibody (10 μg/mL) resulted in a gradual and time-dependent decrease in pSTAT3. On the right panel, densitometry results for pSTAT3 were included.

B) Western blot revealed that SU-DHL-1 cells treated with recombinant IL-22 (10 ng/mL) for 30 minutes increased the level of pSTAT3. Three other signaling proteins in the MAPK kinase pathway (ERK1/2, JNK/SAPK, p38) were also activated. On the right panel, densitometry results for pSTAT3, pJNK/SAPK, pERK1/2, and phoshpho-p38 were included.

C) Compared to the scrambled siRNA, IL-22R1 siRNA downregulated both IL-22R1 and pSTAT3 (cell lysates prepared 24 hours after transfection). The STAT3 and β-actin expression levels were equal in both lanes.