A) RT-PCR studies confirmed the expression of NPM-ALK in Jurkat cells after transfection. Jurkat cells transfected with an empty vector was included as a negative control.
B) Transfection of Jurkat cells with NPM-ALK induced expression of IL-22R1 detectable by Western blots. After stimulation with IL-22 recombinant protein, pSTAT3 levels were detected in Jurkat cells transfected with NPM-ALK, but not in cells transfected with an empty vector.