Protocol for immunization with TSHR A-sub-Ad, blood drawing, and euthanasia. WT and A-subunit tg mice (Lo-tg) were immunized twice (2×) or three times (3×) with TSHR A-sub-Ad. Blood was obtained 4, 10, and 20 weeks after the first immunization. TSHR, thyrotropin receptor; A-sub-Ad, A-subunit adenovirus; WT, wild type; tg, transgenic.