FIG. 2.

TSH binding inhibition (TBI) activity remains detectable 20 weeks after the first immunization in WT (A) and a few tg (B) mice. Mice were immunized twice (2×) or three times (3×) with A-subunit-Ad and sera tested after 4, 10, and 20 weeks. Data for individual mice are shown as black circles for WT mice and as stippled circles for tg mice. Dashed horizontal line: mean+2SD for mice immunized with control adenovirus (16,17). Statistically significant differences: *Tg (2×) 4 weeks versus WT (2×) 4 weeks and WT (3×) 4 weeks, ANOVA, p<0.05; ^#Tg (2×) 10 weeks versus WT (3×) 10 weeks and WT (3×) 4 weeks, ANOVA, p<0.05; **Tg (3×) 20 weeks versus WT (3×) 20 weeks (Rank sum test, p=0.006). SD, standard deviation; ANOVA, analysis of variance; thyrotropin, TSH.