FIG. 3.

TSHR antibody measured by ELISA remains detectable 20 weeks after the first immunization in most WT (A) but in only a few tg (B) mice. Mice were immunized twice (2×) or three times (3×) with A-subunit-Ad and sera tested after 4, 10, and 20 weeks. Data are shown as black circles for individual WT mice and as stippled circles for tg mice. Dashed horizontal line: mean+2SD for mice immunized with control adenovirus (16,17). Statistically significant differences: ^#lower in tg (3×) than in WT (3×) 10 weeks (ANOVA p<0.05); *lower in tg (3×) 4 weeks than in WT (3×) 10 weeks (ANOVA p<0.05). ELISA, enzyme-linked immunosorbent assay.