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. 2012 Jul 12;120(8):1742–1751. doi: 10.1182/blood-2012-03-417139

Figure 5.

Figure 5

Hs2stf/f Tie2Cre+ mice show increased neutrophil infiltration to IL-8 and MIP-2. (A) IL-8 or MIP-2 was injected into preformed dorsal air pouches. After 4 hours, pouches were lavaged and infiltrating neutrophils were quantified by flow cytometry. In a separate experiment, animals were treated with MEL-14 antibody by intravenous injection before injection of IL-8 into the pouch. Each symbol represents a single mouse. ns indicates not significant. (B) Binding of biotinylated IL-8 to wild-type or mutant lung endothelial cells was measured by flow cytometry. Black represents binding to wild-type cells; red, binding to Hs2stf/fTie2Cre+ cells; and blue, binding to Ndst1f/fTie2Cre+. The control sample was incubated only with streptavidin-PE and is shown as filled gray histogram. (C) Binding of MIP-2 to wild-type or mutant lung endothelial cells. (D) Binding of biotinylated FGF-2 to wild-type or mutant lung endothelial cells.