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IL-8 shows enhanced binding to purified heparan sulfate derived from Hs2st-deficient endothelial cells. [35S]Heparan sulfate purified from mutant or wild-type endothelial cells was incubated with biotinylated IL-8 and collected by streptavidin-conjugated magnetic beads. One set of samples was treated with K5 lyases before incubation with IL-8. IL-8 was not added in the control sample.
