FIG. 5.

Trans-membrane motif is critical for the function of KHDC1A. (A) Diagram of KHDC1A, KHDC1B, and KHDC1A-derived truncated constructs used for analysis. (B) HeLa cells were transfected with the constructs indicated. Twenty-four hours after transfection, cells were harvested. The expression of the constructs and the apoptosis were examined by Western blot analysis with indicated antibodies. (C) The cells were transfected and harvested as just described. The cells were fixed, and the apoptosis was quantified by sub-G1 assay. (D) HeLa cells were co-transfected with luciferase reporters and the constructs as indicated. Twenty-four hours after transfection, the cells were harvested, and luciferase assay was performed to determine the protein level of the reporters.