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. Author manuscript; available in PMC: 2013 Aug 17.
Published in final edited form as: Cell. 2012 Aug 17;150(4):842–854. doi: 10.1016/j.cell.2012.07.023

Figure 4. PSMC2Loss cells lack a PSMC2 reservoir.

Figure 4

A. Total PSMC2 levels (top) and Native PAGE immunoblot for PSMA1-6 (middle) in PSMC2Neutral and PSMC2Loss cells. B. Native PAGE immunoblot for PSMA1-6 in A2780 (left) and OVCAR8 (right) after inducible suppression or ectopic expression of PSMC2, respectively. C. Native PAGE 26S and 20S peptidase cleavage in PSMC2Neutral and PSMC2Loss cells. D. Native PAGE 26S and 20S peptidase cleavage in isogenic systems used in B. E. In vitro 26S proteasome activities in PSMC2Neutral and PSMC2Loss cells. Each point represents a cell line; dashed lines represent averages. F. In vitro 26S proteasome activities in isogenic systems used in B and D. G-H. Dose response curve for bortezomib in (G) A2780 cells with and without PSMC2 suppression and (H) OVCAR8 with and without ectopic V5-PSMC2 expression. See also Figure S4 and Table S7.