Table 1. Primer sequences used for real time RT-PCR.

Gene	Forward primer	Reverse primer	Annealing Temp (°C)
SMαA	CACTGTCAGGAATCCTGTGA	CAAAGCCGGCCTTACAGA	65
SM22α	TCTTTGAAGGCAAAGACATGG	TTATGCTCCTGCGCTTTCTT	65
SMMHC	AGATGGTTCTGAGGAGGAAACG	AAAACTGTAGAAAGTTGCTTATTCACT	65
Myocardin	TGCAGCTCCAAATCCTCAGC	TCAGTGGCGTTGAAGAAGAGTT	60
Smoothelin-B	TGCCGAGGCCCAGTGCCTTA	GGGTCCAGCCACGTCTGCTC	60
ACLP	GGCATCGTCAACGGGGCCAA	GCGGTGCACCTGCTCCATGA	60
FRNK	TCGGCTTGGCCCTGAGGACA	GGCGTGAGCAGCAGTCAGCA	60
cTnT	GGAAGAGGCAGACTGAGCGGGA	TCCCGCGGGTCTTGGAGACTT	60
UBC	ATTTGGGTCGCGGTTCTTG	TGCCTTGACATTCTCGATGGT	60
GAPDH	AACAGCCTCAAGATCATCAGC	GGATGATGTTCTGGAGAGCC	60
18S	GACACGGACAGGATTGACAGATTG	TGCCAGAGTCTCGTTCGTTATCG	60
Nestin	GAAACAGCCATAGAGGGCAAA	TGGTTTTCCAGAGTCTTCAGTGA	60
NKX2.5	CAAGTGTGCGTCTGCCTTT	CAGCTCTTTCTTTTCGGCTCTA	60
ISL1	AGATTATATCAGGTTGTACGGGATCA	ACACAGCGGAAACACTCGAT	60
FoxA2	GGGAGCGGTGAAGATGGA	TCATGTTGCTCACGGAGGAGTA	60
Sox17	CGCACGGAATTTGAACAGTA	GGATCAGGGACCTGTCACAC	60
PAX1	CACACTCGGTCAGCAACATC	GGTTTCTCTAGCCCATTCACTG	60
TCF15	GCACCTTCTGCCTCAGCAACCAGC	GGTCCCCCGGTCCCTACACAA	60
MYH2	AGCTCCAAGAACTGTCTCACTCCCA	TGGGCCTCAATGCGCTCCCT	60
MYH4	TGTCTGCTTTGAGCCTGCCACC	TTACAGTAGCTCCAGCTTCGGTCTT	60