Figure 6. Altered energy intake and expenditure in iMGL mice.

(A) Average daily food intake per mouse over the 3 week high fat feeding trial (WT; n = 15, iMGL; n = 20). (B) Time course of average daily food intake per mouse for each week (WT; n = 15, iMGL; n = 15). (C) Time course of oxygen consumption during 24 hr. Values are expressed as ml/kg/min (WT; n = 8, iMGL; n = 8). Note that two-way ANOVA analysis showed a significant difference in genotype (p<0.05 versus WT). (D) Time course of CO₂ production during 24 hr. Values are expressed as ml/kg/min (WT; n = 8, iMGL; n = 8). Two-way ANOVA showed a significant difference in genotype (p<0.05 versus WT). (E) Time course of respiratory quotient (RQ) values (WT; n = 8, iMGL; n = 8). (F) Time course of energy expenditure during 24 hr. Values were expressed as calorie/hour/kg body weight (WT; n = 8, iMGL; n = 8). Two-way ANOVA showed a significant difference in genotype (p<0.05 versus WT). On the X axis, the black bar represents the dark period and the white bar is the light period. (G) Average energy expenditure over 24 h. (H) Average energy expenditure in the dark period. (I) Average energy expenditure in the light period. All indirect calorimetry measurements were collected every minute for 24 hr. (J) Ambulatory activity and (K) Rearing activity in iMGL mice (WT; n = 6, iMGL; n = 6). Gray bars represent activities from dark cycle and white bars for light cycle. Data represent mean ± S.E. *p≤0.05, ** p<0.01 versus wild type littermates.