Skip to main content
. 2012 Aug 28;7(8):e44087. doi: 10.1371/journal.pone.0044087

Figure 6. Characterization of i-SOX2-DAOY medulloblastoma cells.

Figure 6

(A) i-SOX2-DAOY cells were exposed to Dox for 24 hours and nuclear extracts were isolated. A Flag-antibody was used to probe a western blot for the expression of flag-epitope tagged SOX2. (B) MTT assay of i-SOX2-DAOY cells after various duration exposure to Dox. Triplicates of each condition tested were averaged, and the error bars represent standard deviations. ‘*’ and ‘**’ indicates statistical significance (p<0.05 and p<0.005, respectively, student’s t-test). (C) Photomicrographs of i-SOX2-DAOY cells following 48 and 96 hours exposure ± Dox (1 µg/mL). (D) Cell cycle analysis of i-SOX2-DAOY cultured in the absence or presence of Dox (1 µg/mL) for 48 hours. Data for cells without Dox was collected as independent duplicates; whereas, samples with Dox were collected in triplicate. Error bars represent standard deviation. The student’s t-test was used to determine p-values.