. 2012 Aug 28;7(8):e43893. doi: 10.1371/journal.pone.0043893

Table 2. Human parainfluenza viruses real-time reverse-transcription polymerase chain reaction (RT-PCR) panel primer and probe sequences.

Assay, primer/probe	Final concentrationnmol/L	Gene target	Sequence(5′→3′)
HPIV-1^a		HN protein
Forward	900		GTTGTCAATGTCTTAATTCGTATCAATAATT
Reverse	900		GTAGCCTMCCTTCGGCACCTAA
Probe^c	200		TAGGCCAAAGATTGTTGTCGAGACTATTCCAA
HPIV-2^a		HN protein
Forward	900		GCATTTCCAATCTTCAGGACTATGA
Reverse	900		ACCTCCTGGTATAGCAGTGACTGAAC
Probe^c	200		CCATTTACCTAAGTGATGGAATCAATCGCAAA
HPIV-3^b		HN protein
Forward	600		GGAGCATTGTGTCATCTGTC
Reverse	600		TAGTGTGTAATGCAGCTCGT
Probe^c	340		CGCGCTACCCAGTCATAACTTACTCAACAGC AACAGCGCG

Primer and probe sequences for HPIV-1 and HPIV-2 are from F. Watzinger et al. [46].

Primer and probe sequences for HPIV-3 are fromKate E. Templeton et al. [47].

Labeled at the 5′ end with FAM and terminally quenched at the 3′ end with Black Hole Quencher-1.