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. 2012 Aug 28;7(8):e44254. doi: 10.1371/journal.pone.0044254

Figure 5. Up-regulation of p53 by FANCF shRNA and MX in breast cancer cells.

Figure 5

(A) Total cellular proteins (50 μg) from exponentially growing cells treated as indicated in the figure were subjected to western blot analysis with antibodies directed against p53 or p-p53 form as indicated. β-actin was applied as control for equal loading. Cells were pre-treated with p38 inhibitor SB203580 (C) or JNK inhibitor SP600125 (E) for 2 h, and then treated as indicated in the figure were subjected to western blot analysis. (B), (D) and (F) The quantitative analysis of the fold increase of p53 expression in MCF-7 and T-47D cells compared with the controls. P values, # P<0.05 versus transfected with FANCF shRNA and MX in cells. P values, # P<0.05.