Figure 2. Deletion analysis of 1 kb proximal mouse PXR promoter by luciferase reporter gene assays.

(A) Schematic representation of the 1 kb mouse PXR proximal promoter and its various serial deletions. The relative positions of different fragments are indicated. (B) Different PXR promoter luciferase reporter gene constructs were transiently co-transfected along with β-galactosidase into AML-12 cells. After24 hour of expression period, cell lysate was prepared and luciferase and β-galactosidase activities were determined. Levels of relative luciferase activities of different constructs are shown as fold change over the pGL3 basic vector. Data represent the mean ± SE of three different experiments. Asterisks (*) signify luciferase values that differed significantly from the pcDNA transfected cells (P<0.05 in Student's T-test).