Figure 1. siRNA-mediated HRC knock-down (KD) and the expressional profiles of SR proteins in the control and KD samples.

siNC and siHRC oligonucleotides (Dharmacon) were transfected to neonatal rat ventricular cells (NRVCs). After 48 h, transfected NRVCs were solubilized with 1% SDS lysis buffer, and western blot analyses were performed using various antibodies. A: Western blot result of SR proteins in NRVCs after HRC-KD. RyR, ryanodine receptor; HRC, histidine-rich calcium binding protein; SERCA2a, sarcoplasmic reticulum Ca²⁺ ATPase 2a; DHPR, dihydropyridine receptor; NCX, Na⁺-Ca²⁺ exchanger; CSQ, calsequestrin; CaMKII, Ca²⁺/calmodulin-dependent kinase; TRN, triadin. B: Relative expression levels of SR proteins after HRC-KD. siNC, negative control of knock-down oligonucleotide; siHRC, HRC-KD oligonucleotide (**P<0.01). Note that there were no expressional changes of other SR proteins by knock-down of HRC.