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. 2012 Aug 28;7(8):e43282. doi: 10.1371/journal.pone.0043282

Figure 4. [3H]-ryanodine binding and oxalate-supported Ca2+ uptake assays.

Figure 4

After 48 h of siRNA transfection, the NRVCs were used for [3H]-ryanodine binding and oxalate-supported Ca2+ uptake assays. A: [3H]-ryanodine binding assay results using siNC and siHRC transfected NRVCs. The significantly different Bmax values of [3H]-ryanodine binding were 0.146±0.011 and 0.224±0.03 pmol/mg for siNC (○) and siHRC (•) samples, respectively. The Kd values are not significantly different between the 2 samples (siNC: 2.786±0.664 nM vs. siHRC: 3.052±0.702 nM). B: Oxalate-supported Ca2+ uptake assay results. Oxalate-supported SR-based Ca2+ uptake in siNC (○) and siHRC (•) transfected NRVCs were determined at 1, 2, 3 and 4 min. The rates of Ca2+ uptake were 57.89±2.54 and 70.11±2.26 nmol/mg/4 min for siNC and siHRC samples, respectively. 7 sets of siNC and siHRC NRVCs were used for statistical analyses (*P value <0.05).