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. 2012 Aug 28;7(8):e43282. doi: 10.1371/journal.pone.0043282

Figure 7. Increased apoptosis in HRC-KD heart under TAC-HF.

Figure 7

TUNEL assay was performed using paraffin-embedded heart sections. A: Results of TUNEL assay. Left, white arrow heads show TUNEL positive pink signal. Right, relative numbers of TUNEL positive cardiomyocyte nuclei (shNC sham: 1±0.46; shNC failure: 4.38±0.21; shHRC sham: 1.6±0.4; shHRC failure: 5.44±0.17). B: Upper, western blot results of caspase 3, cleaved caspase 3, Bax and Bcl-2 from shNC sham, shNC failure, shHRC sham, and shHRC failure mouse hearts. Lower, summary of Bax per Bcl-2 ratio and the expressional changes of the apoptosis marker, cleaved caspase 3 (*P<0.05, **P<0.01).