Figure 8. Investigation of the signaling cascade responsible for the enhanced apoptosis by HRC-KD in TAC-HF model.

A: Western blot assay results. Heart lysates from shNC sham, shNC failure, shHRC sham, and shHRC failure mouse hearts were probed with antibodies against various target proteins involved in the apoptosis signaling pathway and Ca²⁺ cycling. P-CaMKII, phosphorylated CaMKII; P-p38 MAPK, phosphorylated p38 MAPK; PLB, phospholamban; P-PLB, phosphorylated phospholamban. B: Relative expression of differentially expressed proteins in shNC sham, shNC failure, shHRC sham, and shHRC failure heart samples (*P<0.05, **P<0.01, ***P<0.001).