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. 2012 Aug 28;7(8):e41731. doi: 10.1371/journal.pone.0041731

Figure 2. SEC analysis of AG22 (1–422) S16D/S411D and 14-3-3.

Figure 2

A. SEC elution profiles of the cross-linked (black) and uncross-linked (gray) complexes. The cross-linked complex of AG22 (1–422) S16D/S411D and 14-3-3 eluted at 12 mL from a Superdex S200 (10/300 GL) column, while the uncross-linked complex eluted at 13.5 mL. Absorbance (mAU) at 280 nm was monitored. Elution volumes of molecular mass standards are indicated at the top of the panel. B. Elution fractions from gel filtration were analyzed by SDS-PAGE and stained with Coomassie Blue.