Table 1. Detection of H5N1 challenge virus in tracheal and cloacal swabs of vaccinated chickens.
| ON/IT vaccinationa | IM vaccination | ||||||||||||||
| NDV-H5 | NDV-sH53 | NDV-H5 | NDV-sH53 | sH53 | |||||||||||
| Bird # | D2 | D4 | D7 | D2 | D4 | D7 | D2 | D4 | D7 | D2 | D4 | D7 | D2 | D4 | D7 |
| 1 | 2.3 | − | − | 1.9 | − | − | 3.6 | − | − | 5.0 | − | − | − | − | − |
| 2 | − | 2.5 | − | x | x | x | 3.9 | 2.7 | − | 3.1 | − | − | − | − | − |
| 3 | 3.9 | 5.7 | x | 2.3 | − | − | 4.1 | − | − | − | 4.6 | − | − | − | − |
| 4 | 1.6 | − | − | x | x | x | − | − | − | 3.7 | 2.8 | − | − | − | − |
| 5 | 4.0 | 4.0 | x | x | x | x | 3.3 | − | − | 3.3 | − | − | − | − | − |
| 6 | 2.8 | 3.5 | − | x | x | x | 4.6 | 2.9 | − | 2.8 | − | − | 1.6 | − | − |
| 7 | − | 4.4 | − | x | x | x | 4.2 | − | − | − | 1.2 | − | − | − | − |
| 8 | 4.4 | 4.0 | − | 2.7 | − | − | 3.2 | 4.5 | − | − | − | − | 4.2 | − | − |
| 9 | − | 1.6 | − | 1.8 | − | − | 3.5 | 1.8 | − | − | 1.8 | − | − | − | − |
| 10 | 2.6 | 3.4 | − | − | − | − | 3.6 | 5.3 | − | − | 5.8 | − | − | − | − |
a
Day (D) post infection on which tracheal and cloacal swabs were collected are indicated. Amount of RNA detected in swabs is expressed as log10 TCID50 ml-1 equivalents.− = negative (CT >39.0); x = not tested because chicken did not survive after challenge with HPAIV H5N1.