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. 2012 Aug 28;7(8):e43494. doi: 10.1371/journal.pone.0043494

Figure 4. Hydrolysis of nid-2 by catS.

Figure 4

(A) Western-blot of human nid-2 incubated with catS, catB, catL and catK at pH 5.5 for 30 min (enzyme:substrate ratio = 1∶143, w/w). (B) Densitometry analysis of nid-2 after incubation with catS (1, 5, 10, 30 min) at pH 5.5 (white bar) and pH 7.4 (grey bar). E-64 inactivated catS was used as a control. The percentage of residual nid-2 was expressed as means ± S.D (*, P<0.05 and **, P<0.01 when compared to control). A representative of three independent western blots at both pH is shown.