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. 2012 Aug 28;7(8):e43494. doi: 10.1371/journal.pone.0043494

Figure 5. Influence of catS on nid-1 binding to BM constituents.

Figure 5

(A) ELISA results showing the binding at pH 5.5 of undigested nid-1 (0–250 ng) to immobilized ligands (500 ng): laminin-211/221 (black diamond), collagen IV (dark grey square) and perlecan (white triangle). (B) ELISA results showing the binding at pH 5.5 of nid-1 (25 ng) pre-incubated with catS (5 nM) for 5–60 min to immobilized ligands (500 ng): laminin-211/221 (black bar), collagen IV (dark grey bar) and perlecan (white bar). Controls were performed using undigested nid-1 (25 ng) at pH 5.5. (C) The same experiment was repeated at pH 7.4. Experiments were performed three independent times (*, P<0.05 and **, P<0.01 when compared to control).