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. 2012 Aug 28;7(8):e43709. doi: 10.1371/journal.pone.0043709

Figure 2. LPS-dependent increase in cytokines and chemokines ex vivo.

Figure 2

Ascending TNF-α (A) and intracellular MIP-1β (B) production after 24-hour incubation with increasing concentrations of LPS in marmoset PCLS. LPS-induced increase in TNF-α (C) and intracellular MIP-1β (D) in marmoset PCLS was significantly suppressed by dexamethasone (dxm). Marmoset PCLS and marmoset WBA (E) on the one hand and marmoset PCLS and human PCLS (F) on the other hand showed significant correlations for TNF-α secretion (Spearman’s rank correlation coefficient (rS)  = 1.0 with p  = 0.0004, and rs  = 0.9 with p  = 0.01, respectively). Symbols: ○: 2.5 ng/mL LPS, □: 5 ng/mL LPS, ▾: 10 ng/mL LPS, ▵: 100 ng/mL LPS, ◊: 250 ng/mL LPS, x: 500 ng/mL LPS. Data are presented as mean ± SEM, *p<0.05, **p<0.01, Mann-Whitney test (TNF-α: n  = 6, MIP-1β: n  = 4). Correlations were evaluated using a linear regression analysis model combined with Spearman’s rank correlation coefficient. NHP  =  non-human primate, int  =  intracellular.