Table 4. Performance of different methods of specimen preparation in relation to DNA extraction success.
| Method | Duration of drying | Median DNA yield (ng) | Median DNA purity (A260/A280) | PCR success: No of positive amplicons (%) | |||
| rbcL | LEAFY | P6 loop | |||||
| 1. | Silica | 1 day | 1312 | 1.866 | 18 (100) | 18 (100) | 18 (100) |
| 2. | Air (heat) | 12 hours | 1322 | 1.894 | 14 (77.8) | 13 (72.2) | 18 (100) |
| 3. | Air (room temperature) | 2 weeks | 967 | 1.895 | 15 (83.3) | 16 (88.9) | 18 (100) |
| 4. | Alcohol (quick) | Overnight | 652 | 1.979 | 11 (61.1) | 13 (72.2) | 18 (100) |
| 5. | Alcohol (slow) | 2 weeks | 533 | 1.887 | 11 (61.1) | 11 (61.1) | 17 (94.4) |