Figure 4. PNA array analysis of Cy5-labeled oligonucleotides (point mutations present in Ogliarola leccese) (headings refer to PNA probes deposited). (A) DNA T60; (B) DNA A60; (C) DNA C198; (D) DNA G198 with washing at room temperature; (E) G198 after washing at 60°C. Oligonucleotides concentrations were 0.5 µM; hybridization was performed in SSC 4x with 0.1%, SDS at 40°C for 2 h. Scan was performed at λ_ex = 646 nm, λ_em = 664 nm; laser power 100%, gain 40%. Spots in each column represent replicates.