Figure 8. Results obtained with DNA extracted from extra virgin olive oil samples. (A) Agarose gel analysis of the 296 bp PCR product from (1) Canino and (2) Ogliarola leccese, and of asymmetric PCR from Canino (3 and 5, replicates) and Ogliarola leccese (4 and 6, replicates); M 50 bp ladder marker; (B) PNA array analysis of the 296 bp DNA obtained by asymmetric PCR shown in (A); (C) PNA array analysis of DNA extracted from oil of the Canino cultivar using nested PCR and achiral PNA; (D) PNA array analysis obtained using chiral PNA (ch-A60 and ch-T60) in comparison with achiral PNA (A60, T60) with DNA extracted from Ogliarola leccese oil. Spots in each column of the PNA arrays represent replicates.