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. 2012 Apr 1;3(2):63–72. doi: 10.4161/adna.20603

Table 3. Melting temperatures for the PNA synthesized with full match and singly mismatched DNA measured in PBS buffer.

PNA Tm fullmatch Tm mismatch ΔTm
PNA-A60
 54°C
 35°C
 19°C
PNA-T60
 46°C
 38°C
 8°C
PNA-ch-A60
 55°C
 35°C
 20°C
PNA-ch-t60
 48°C
 38°C
 10°C
PNA-C198
 81°C
 65°C
 16°C
PNA-G198 85°C 70°C 15°C

c = 5 μM for each strand.