Figure 5. ATG12 and BECN1 are novel targets of MIR30B. (A) The region of the human ATG12 and BECN1 mRNA 3′UTR predicted to be targeted by MIR30B (TargetScan 4.2). (B and C) Representative fluorescent microscopic image and flow cytometry results demonstrate that GFP expression of the GFP-ATG12 and BECN1 reporters was inhibited by MIR30B. HEK-293 cells were co-transfected with the GFP reporter vectors and compared with cells transfected with a mimic or control of MIR30B. (D) Luciferase reporter assay. HEK293 cells were transiently co-transfected with luciferase reporter vectors and either MIR30B mimic or control. Luciferase activity was normalized to the activity of Renilla luciferase. (E and F) AGS cells were transiently transfected with MIR30B mimic, inhibitor or control for 24 h. The mRNA and protein levels of BECN1 and ATG12 were determined by qRT-PCR and western blot. Data are representative of five independent experiments (* p < 0.05).