Figure 5. TP53 is essential for the autophagy induction by ERAL1 knockdown. (A) HCT-116 TP53^+/+ and HCT-116 TP53^−/− cells were transfected with ERAL1 siRNA. At 72 h post-siRNA transfection, cells were subjected to western blotting to detect the levels of the indicated proteins. (B) HCT-116 TP53^+/+ and HCT-116 TP53^−/− cells were transfected with GFP-LC3 plasmid and then treated with control siRNA or ERAL1 siRNA, respectively. At 72 h post-siRNA transfection, GFP-LC3 puncta formation in the cells was detected by confocal microscopy. The percentage of GFP-LC3 puncta-positive cells was quantified as described under Materials and Methods. Representative data were from three independent experiments. The p value derived from Student’s t-test is **p < 0.001.