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. 2012 Aug 1;1(5):600–608. doi: 10.4161/onci.20225

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Copyright © 2012 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name onci-1-600-g4.jpg — Figure 4. Impaired thymocyte development in Vav1^−/− M-MuLV mice. A) Flow cytometric analysis of thymi from control and M-MuLV infected mice. Cells were stained with CD44 and CD25 antibodies to distinguish DN1 (CD44⁺/CD25^-), DN2 (CD44⁺/CD25⁺), DN3 (CD44^-/CD25⁺) and DN4 (CD44^-/CD25^-) subsets within the CD4-CD8- population. B) Percentages of DN1, DN2, DN3 and DN4 from representative populations of mice (Vav1^+/+ control (n = 6); Vav1^−/− control (n = 6); Vav1^+/+ M-MuLV (n = 4); Vav1^−/− M-MuLV (n = 9)). The numbers in brackets reflect the mice whose plots are depicted in panel A. Data were evaluated using Student’s t-test: * p < 0.05; **p < 0.01; ***p < 0.001.