Figure 6. IL-8 secretion by autophagy inhibition in CSE-induced senescent HBEC. For preparation of the conditioned medium, HBEC were treated with CSE (1.0%) in the absence or presence of Torin1 (250 nM) and bafilomycin A (200 nM) for 24h, washed three times with PBS, then incubated in fresh BEGM for 48h. (A) ELISA showing IL-8 in conditioned media with or without CSE-treatment in the absence (lane 1, 2), or presence of Torin1 (lane 3, 4) and bafilomycin A (lane 5, 6). Shown is the arbitrary units ± SEM *p < 0.05. **p < 0.001. (B) ELISA showing IL-8 in conditioned media from control siRNA (lane 1, 2) or LC3B siRNA (lane 3, 4) transfected HBEC. CSE (1.0% for 48 h) treatment was started 24 h post-siRNA transfection. Shown is the arbitrary units ± SEM *p < 0.05.